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rabbit polyclonal ab against vegfa  (Proteintech)


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    Structured Review

    Proteintech rabbit polyclonal ab against vegfa
    Genes regulated by ZEB1.
    Rabbit Polyclonal Ab Against Vegfa, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 794 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal ab against vegfa/product/Proteintech
    Average 96 stars, based on 794 article reviews
    rabbit polyclonal ab against vegfa - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer"

    Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0148774

    Genes regulated by ZEB1.
    Figure Legend Snippet: Genes regulated by ZEB1.

    Techniques Used: Control

    (A) MDA-MB-231 cells were transiently transfected with human ZEB1 expression plasmid or empty vector control. At the indicated time points, expression of ZEB1, VEGFA and VEGFC was verified by Western blotting. Actin was used as a loading control. (B) Production of VEGFA and VEGFC protein were verified by ELISA at the indicated time points following ZEB1 overexpression. * P < 0.05, ** P < 0.01 vs respective control in one-way ANOVA followed by Tukey’s Honestly Significant Difference test. (C) HUVECs cultured in the presence or absence of VEGFA (20 and 40 ng/mL) or anti-VEGFA neutralized Ab (1 μg/mL) along with ZEB1/231-derived conditioned medium were subjected to a tube formation assay and photographed. (D) Quantification of tube formation was expressed as length of capillary tubes formed per mm 2 . * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.
    Figure Legend Snippet: (A) MDA-MB-231 cells were transiently transfected with human ZEB1 expression plasmid or empty vector control. At the indicated time points, expression of ZEB1, VEGFA and VEGFC was verified by Western blotting. Actin was used as a loading control. (B) Production of VEGFA and VEGFC protein were verified by ELISA at the indicated time points following ZEB1 overexpression. * P < 0.05, ** P < 0.01 vs respective control in one-way ANOVA followed by Tukey’s Honestly Significant Difference test. (C) HUVECs cultured in the presence or absence of VEGFA (20 and 40 ng/mL) or anti-VEGFA neutralized Ab (1 μg/mL) along with ZEB1/231-derived conditioned medium were subjected to a tube formation assay and photographed. (D) Quantification of tube formation was expressed as length of capillary tubes formed per mm 2 . * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Over Expression, Cell Culture, Derivative Assay, Tube Formation Assay

    Positive correlation between ZEB1 and VEGFA expression in breast cancer.
    Figure Legend Snippet: Positive correlation between ZEB1 and VEGFA expression in breast cancer.

    Techniques Used: Expressing

    The non-negative percentage analysis for VEGFA (A) and CD31 (B) indicates a positive correlation with ZEB1 expression in breast cancer tumors from 228 subjects. (C) Representative images of immunohistochemical staining of ZEB1, VEGFA, and CD31 in tumors from 4 cases are shown. Scale bars, 25 μm.
    Figure Legend Snippet: The non-negative percentage analysis for VEGFA (A) and CD31 (B) indicates a positive correlation with ZEB1 expression in breast cancer tumors from 228 subjects. (C) Representative images of immunohistochemical staining of ZEB1, VEGFA, and CD31 in tumors from 4 cases are shown. Scale bars, 25 μm.

    Techniques Used: Expressing, Immunohistochemical staining, Staining

    MDA-MB-231 cells were transiently transfected with the human ZEB1 expression plasmid or empty vector control, followed by treatment with PI-103 (10 μM) or SB203580 (10 μM). At the indicated time points, upregulation of VEGFA expression were verified by qPCR (A and B), Western blotting (C and D) and ELISA (E and F) in ZEB1-expressing versus control cells. GAPDH and actin were used to normalize VEGFA levels. * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.
    Figure Legend Snippet: MDA-MB-231 cells were transiently transfected with the human ZEB1 expression plasmid or empty vector control, followed by treatment with PI-103 (10 μM) or SB203580 (10 μM). At the indicated time points, upregulation of VEGFA expression were verified by qPCR (A and B), Western blotting (C and D) and ELISA (E and F) in ZEB1-expressing versus control cells. GAPDH and actin were used to normalize VEGFA levels. * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Control, Western Blot, Enzyme-linked Immunosorbent Assay

    (A) Sequential deletion and mutation of SP1 elements on the human VEGFA promoter were fused to the luciferase reporter. (B) MDA-MB-231 cells were co-transfected with the ZEB1 expression plasmid (1 μg/well) and different wild-type VEGFA promoter luciferase reporter constructs (1 μg/well). Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values were normalized to Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (C) MDA-MB-231 cells were co-transfected with ZEB1 expression plasmid and wild-type or mutant VEGFA promoter luciferase reporters. Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values are normalized with Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (D) The association of SP1 with the proximal human VEGFA promoter was analyzed by ChIP assay, using polyclonal Ab against SP1 or unrelated IgG Ab. The amplified sequence of the VEGFA promoter fragment containing SP1 elements is shown. Input DNA amounts were confirmed by equal loading of chromatin. (E) Overexpression of ZEB1 significantly enhanced the recruitment of SP1 to the endogenous VEGFA promoter as confirmed by a quantitative ChIP assay. * P < 0.05 vs respective control in Student’s t -test.
    Figure Legend Snippet: (A) Sequential deletion and mutation of SP1 elements on the human VEGFA promoter were fused to the luciferase reporter. (B) MDA-MB-231 cells were co-transfected with the ZEB1 expression plasmid (1 μg/well) and different wild-type VEGFA promoter luciferase reporter constructs (1 μg/well). Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values were normalized to Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (C) MDA-MB-231 cells were co-transfected with ZEB1 expression plasmid and wild-type or mutant VEGFA promoter luciferase reporters. Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values are normalized with Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (D) The association of SP1 with the proximal human VEGFA promoter was analyzed by ChIP assay, using polyclonal Ab against SP1 or unrelated IgG Ab. The amplified sequence of the VEGFA promoter fragment containing SP1 elements is shown. Input DNA amounts were confirmed by equal loading of chromatin. (E) Overexpression of ZEB1 significantly enhanced the recruitment of SP1 to the endogenous VEGFA promoter as confirmed by a quantitative ChIP assay. * P < 0.05 vs respective control in Student’s t -test.

    Techniques Used: Mutagenesis, Luciferase, Transfection, Expressing, Plasmid Preparation, Construct, Control, Amplification, Sequencing, Over Expression



    Similar Products

    rabbit polyclonal ab against vegfa  (Proteintech)
    96
    Proteintech rabbit polyclonal ab against vegfa
    Genes regulated by ZEB1.
    Rabbit Polyclonal Ab Against Vegfa, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal ab against vegfa/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal ab against vegfa - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Genes regulated by ZEB1.

    Journal: PLoS ONE

    Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

    doi: 10.1371/journal.pone.0148774

    Figure Lengend Snippet: Genes regulated by ZEB1.

    Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

    Techniques: Control

    (A) MDA-MB-231 cells were transiently transfected with human ZEB1 expression plasmid or empty vector control. At the indicated time points, expression of ZEB1, VEGFA and VEGFC was verified by Western blotting. Actin was used as a loading control. (B) Production of VEGFA and VEGFC protein were verified by ELISA at the indicated time points following ZEB1 overexpression. * P < 0.05, ** P < 0.01 vs respective control in one-way ANOVA followed by Tukey’s Honestly Significant Difference test. (C) HUVECs cultured in the presence or absence of VEGFA (20 and 40 ng/mL) or anti-VEGFA neutralized Ab (1 μg/mL) along with ZEB1/231-derived conditioned medium were subjected to a tube formation assay and photographed. (D) Quantification of tube formation was expressed as length of capillary tubes formed per mm 2 . * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.

    Journal: PLoS ONE

    Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

    doi: 10.1371/journal.pone.0148774

    Figure Lengend Snippet: (A) MDA-MB-231 cells were transiently transfected with human ZEB1 expression plasmid or empty vector control. At the indicated time points, expression of ZEB1, VEGFA and VEGFC was verified by Western blotting. Actin was used as a loading control. (B) Production of VEGFA and VEGFC protein were verified by ELISA at the indicated time points following ZEB1 overexpression. * P < 0.05, ** P < 0.01 vs respective control in one-way ANOVA followed by Tukey’s Honestly Significant Difference test. (C) HUVECs cultured in the presence or absence of VEGFA (20 and 40 ng/mL) or anti-VEGFA neutralized Ab (1 μg/mL) along with ZEB1/231-derived conditioned medium were subjected to a tube formation assay and photographed. (D) Quantification of tube formation was expressed as length of capillary tubes formed per mm 2 . * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.

    Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

    Techniques: Transfection, Expressing, Plasmid Preparation, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Over Expression, Cell Culture, Derivative Assay, Tube Formation Assay

    Positive correlation between ZEB1 and VEGFA expression in breast cancer.

    Journal: PLoS ONE

    Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

    doi: 10.1371/journal.pone.0148774

    Figure Lengend Snippet: Positive correlation between ZEB1 and VEGFA expression in breast cancer.

    Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

    Techniques: Expressing

    The non-negative percentage analysis for VEGFA (A) and CD31 (B) indicates a positive correlation with ZEB1 expression in breast cancer tumors from 228 subjects. (C) Representative images of immunohistochemical staining of ZEB1, VEGFA, and CD31 in tumors from 4 cases are shown. Scale bars, 25 μm.

    Journal: PLoS ONE

    Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

    doi: 10.1371/journal.pone.0148774

    Figure Lengend Snippet: The non-negative percentage analysis for VEGFA (A) and CD31 (B) indicates a positive correlation with ZEB1 expression in breast cancer tumors from 228 subjects. (C) Representative images of immunohistochemical staining of ZEB1, VEGFA, and CD31 in tumors from 4 cases are shown. Scale bars, 25 μm.

    Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

    Techniques: Expressing, Immunohistochemical staining, Staining

    MDA-MB-231 cells were transiently transfected with the human ZEB1 expression plasmid or empty vector control, followed by treatment with PI-103 (10 μM) or SB203580 (10 μM). At the indicated time points, upregulation of VEGFA expression were verified by qPCR (A and B), Western blotting (C and D) and ELISA (E and F) in ZEB1-expressing versus control cells. GAPDH and actin were used to normalize VEGFA levels. * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.

    Journal: PLoS ONE

    Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

    doi: 10.1371/journal.pone.0148774

    Figure Lengend Snippet: MDA-MB-231 cells were transiently transfected with the human ZEB1 expression plasmid or empty vector control, followed by treatment with PI-103 (10 μM) or SB203580 (10 μM). At the indicated time points, upregulation of VEGFA expression were verified by qPCR (A and B), Western blotting (C and D) and ELISA (E and F) in ZEB1-expressing versus control cells. GAPDH and actin were used to normalize VEGFA levels. * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.

    Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

    Techniques: Transfection, Expressing, Plasmid Preparation, Control, Western Blot, Enzyme-linked Immunosorbent Assay

    (A) Sequential deletion and mutation of SP1 elements on the human VEGFA promoter were fused to the luciferase reporter. (B) MDA-MB-231 cells were co-transfected with the ZEB1 expression plasmid (1 μg/well) and different wild-type VEGFA promoter luciferase reporter constructs (1 μg/well). Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values were normalized to Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (C) MDA-MB-231 cells were co-transfected with ZEB1 expression plasmid and wild-type or mutant VEGFA promoter luciferase reporters. Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values are normalized with Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (D) The association of SP1 with the proximal human VEGFA promoter was analyzed by ChIP assay, using polyclonal Ab against SP1 or unrelated IgG Ab. The amplified sequence of the VEGFA promoter fragment containing SP1 elements is shown. Input DNA amounts were confirmed by equal loading of chromatin. (E) Overexpression of ZEB1 significantly enhanced the recruitment of SP1 to the endogenous VEGFA promoter as confirmed by a quantitative ChIP assay. * P < 0.05 vs respective control in Student’s t -test.

    Journal: PLoS ONE

    Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

    doi: 10.1371/journal.pone.0148774

    Figure Lengend Snippet: (A) Sequential deletion and mutation of SP1 elements on the human VEGFA promoter were fused to the luciferase reporter. (B) MDA-MB-231 cells were co-transfected with the ZEB1 expression plasmid (1 μg/well) and different wild-type VEGFA promoter luciferase reporter constructs (1 μg/well). Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values were normalized to Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (C) MDA-MB-231 cells were co-transfected with ZEB1 expression plasmid and wild-type or mutant VEGFA promoter luciferase reporters. Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values are normalized with Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (D) The association of SP1 with the proximal human VEGFA promoter was analyzed by ChIP assay, using polyclonal Ab against SP1 or unrelated IgG Ab. The amplified sequence of the VEGFA promoter fragment containing SP1 elements is shown. Input DNA amounts were confirmed by equal loading of chromatin. (E) Overexpression of ZEB1 significantly enhanced the recruitment of SP1 to the endogenous VEGFA promoter as confirmed by a quantitative ChIP assay. * P < 0.05 vs respective control in Student’s t -test.

    Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

    Techniques: Mutagenesis, Luciferase, Transfection, Expressing, Plasmid Preparation, Construct, Control, Amplification, Sequencing, Over Expression